Experimental study on general pharmacological effects of turmeric extract

The main component of the Alcohol extract of turmeric is a phenolic pigment , which mainly includes curcumin, demethoxycurcumin, bisdemethoxycurcumin and the like. Experiments have shown that turmeric extract has anti-lipid, anti-oxidation, fibrinolysis, inhibition of cyclooxygenase and lipoxygenase, and anti-tumor. The authors studied the general pharmacological effects of turmeric extract on experimental animals , and the results are reported below. 1 Materials and methods

1.1 Test drug The turmeric extract was provided by the Institute of Pharmaceutical and Biotechnology of Chengdu High-tech Zone. Batch number : 970501, purity : containing 58% curcumin. The drug has a yellow fine powder and no characteristic odor. When it is used , weigh a certain amount with an electronic analytical balance , and mix it with 0.1% CMC in a mortar to prepare a suitable suspension.

. N IH 12 animals 30 mice, weighing 18 ~ 22g; 90 Wistar rats weighing 270 ~ 310g. Both male and female , provided by the school . The animal certification numbers are : medical words 310101008 and 310101011 . All experimental animals in the laboratory

Feed for 3 days under conditions to accommodate laboratory conditions.

1.3 method

1. 3. Effect of extracts of turmeric. 1 N IH neuropsychiatric mice 30 mice, were randomly divided into three groups, i.e., 513mg / kg group, 171 mg / kg group and 0. 5% CMC group. Before and after administration using parallel control method, apparatus, and CS22 pole climbing mice spontaneous activity meter (Medical drugs developed) with self administration in mice before dosing and after 30 min, 90 min and 180 min were observed in each rat balance and coordination and locomotor activity (2 min) change

1. 3. 2 Turmeric extract ig affecting respiratory rate and respiratory amplitude Wistar anesthetized rats 30 rats were randomly divided into three groups, i.e., 270 mg / kg group, 67. 5 mg / kg group And 0.5% CMC group. And front and rear parallel controlled self-administration method to clamp onto the frog heart beneath the skin of rats xiphoid and recorded before 30 min after administration and administration with diethyl polygraph, 90 min and 180 min each mouse respiratory rate And magnitude

1 . 3 . 3 The effect of turmeric extract ig on blood pressure of non-anesthetized rats The number and group of rats were the same as 1. 3 . And front and rear parallel controlled self-administration method, RB with rat tail type P21 pressure rate-pressure meter, measured before administration 30 min, 90 min and 180 min each rat and the blood pressure variation value after administration

1 . 3 . 4 The effect of turmeric extract ig on the electrocardiogram of anesthetized rats The number and group of rats were the same as 1. 3. 2 . In the use of 3% sodium pentobarbital 30mg / kg ip anesthetized rats before and after administration and their parallel control method, using electrocardiograph (Shanghai yield) was measured before administration and after 30 min administration, 90 min and 180 min Large Changes in the values ​​of the various parameters of the mouse ECG.

1.4 Statistical processing All data were expressed as (x ± s ), and the data were subjected to inter-group F test statistical processing using SAS statistical software .

2 results

The effect of turmeric extract on the nervous system of mice showed that 513 mg/kg and 171 mg/kg ig had no significant effect on the spontaneous activity of mice ; the climbing experiment showed that the turmeric extract had no obvious balance coordination ability in mice. influences.

2.2 The effect of turmeric extract ig on blood pressure of non-anesthetized rats showed that 270 mg/kg and 67.5 mg/kg ig had no significant effect on blood pressure in conscious rats.

2.3 The effect of turmeric extract ig on respiratory rate and respiratory amplitude of anesthetized rats showed that 270 mg/kg and 66.5 mg/kg ig had no significant effect on respiratory rate and respiratory amplitude of anesthetized rats.