Principle and procedure of chemiluminescence immunoassay kit for human B cell activating factor (BAFF/CD257)
product description: Fundamental: Steps:
China manufacturers ,suppliers of Apis Raw,Naphazoline Powder Benefits,Naphazoline Hcl Powder,Neomycine Pharmaceutical API, China manufacturers ,suppliers of Apis Raw,Naphazoline Powder Benefits,Naphazoline Hcl Powder,Neomycine Pharmaceutical API Xi'an Henrikang Biotech Co.,Ltd , https://www.henrikangbio.com English name  Human BAFF/CD257 (B-Cell Activating Factor) CLIA Kit  Chinese name   Human B cell activating factor (BAFF/CD257) chemiluminescence immunoassay kit   Item number  E-CL-H0009c  species  Human / person  specification  96T/Kit (8*12 strips) 48T/Kit (8*6 strips)  Detection method   Double antibody sandwich method   examination range  62.5~4000pg/mL  Sensitivity  37.5pg/mL
This kit uses a double antibody sandwich method. The anti-Human BAFF/CD257 antibody was coated on the microtiter plate, and the BAFF/CD257 in the specimen or standard was bound to the coated antibody, and the free component was washed away. Biotinylated anti-Human BAFF/CD257 antibody and horseradish peroxidase-labeled avidin were sequentially added. The anti-Human BAFF/CD257 antibody binds to Human BAFF/CD257 bound to the coated antibody, biotin binds to avidin to form an immune complex, and the free component is washed away. The luminescent substrate mixture was added, the luminescent substrate was fluoresced under the catalysis of horseradish peroxidase, and the chemiluminescence value (CL) was measured by a chemiluminescence immunoassay, and the BAFF/CD257 concentration was positively correlated with the chemiluminescence value. The concentration of BAFF/CD257 in the specimen was determined by plotting a standard curve.
1. Add 100 μL of standard or sample to each well and incubate at 37 ° C for 90 minutes.
2. Pour the liquid in the well, pat dry, add 100 μL of biotinylated antibody working solution, incubate at 37 ° C for 60 minutes.
3. Wash 3 times
4. Add 100 μL of enzyme conjugate working solution and incubate at 37 ° C for 30 minutes.
5. Wash 5 times
6. Add 100 μL of substrate solution and incubate for 5 minutes at 37 °C
7. Readings
8. Calculation of results
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