Affinity chromatography principle and application introduction

Affinity chromatography is a special type of chromatographic technique that utilizes a specific affinity between a substance to be separated and its specific ligand to achieve separation.
Biomolecule pairs with specific affinity are: antigens and antibodies, DNA and complementary DNA or RNA, enzymes and its substrates or competitive inhibitors, hormones (or drugs) and their receptors, vitamins and their specificity Binding proteins, glycoproteins and their corresponding plant lectins. One of the pair of affinity molecules is linked to the insoluble carrier as a stationary phase adsorbent, and when the sample containing the mixed component passes through the stationary phase, only a substance having a specific affinity with the stationary phase molecule can Adhesively bound by the stationary phase, other unrelated components with no affinity flow out with the mobile phase, then change the flow component and elute the bound affinity. The carrier used in affinity chromatography is referred to as a matrix, and the compound covalently attached to the matrix is ​​referred to as a ligand.
The affinity chromatography purification process is simple and rapid, and the separation efficiency is high. It is especially effective for the separation of very little and unstable active substances. However, this method must be directed to a separate object, to prepare a specific ligand and to seek stable conditions for chromatography.
The affinity chromatography method can be used to separate and purify the target protein from the heteroprotein solution simply, quickly and efficiently. Affinity chromatography has become a widely used protein separation technology, or can provide antibodies or An anti-serum was prepared for the affinity chromatography column.
Affinity chromatography can be used for: 1. Purification of biomacromolecules 2. Concentration of dilute solutions 3. Storage of unstable proteins 4. Removal of residual contaminants from purified molecules 5. Adsorption purification with immunosorbent No Complementary ligands for biomacromolecules 6. Separation of nucleic acids is an important aspect of affinity chromatography applications.
The antibodies in our company catalog can be prepared into affinity chromatography columns according to the customer's requirements, or the affinity chromatography column can be prepared for the antibody or antiserum provided by the customer.

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