Hole experiment / orifice plate experiment / hole plate experiment operation steps and notes
Experiment hole / well plate experiments / hole-board test (the holeboard test) A basic principle The well plate experiment was first established by Boissiex and Simon in 1962 and has since been widely used for pharmacodynamic studies. The experiment uses the two factors of curiosity and fear to control the behavior of animals in the new environment, and uses escapes to reflect the effects of these two factors. The animal's repeated dipping reflects its novelty and desire to escape. It is generally believed that the drug increases the number and time of the drill bit at a dose that does not affect the animal's locomotor activity, and exhibits an anxiolytic effect. The reduction in the number and timing of the drill bit is an anxiety-causing effect. II. Procedure The experimental setup of rats and mice is different. The rat device is a wooden box of 66cm x 56cm x 47cm. The bottom plate has four large round holes with a diameter of 3.8cm and a depth of 1cm. The two holes are 14cm away from the nearest wall and the other two holes are 17cm. 12em; the mouse wooden box is 44cm x 40cm x 27cm, the diameter of each of the four holes is 3cm, the thickness is 1.8cm, and the distance from the center of each hole to the nearest wall is l0cm. After the 1980s, infrared photocells were installed on the wall of the orifice box and around each hole, and connected with the microcomputer, so that the number of drill bits and bit time, movement activity and standing number of the animal can be automatically recorded at the same time. Improve work efficiency and increase the reliability of the experiment. Male adult rats or mice were used for the experiment. Place the animal in the center of the well plate, facing away from the viewer. The animal's eyes disappeared into the hole as a bit (a head-dip), recording the number of drill bits for 5-10 min and the duration of the drill bit. III . Notes and evaluation (1) There is no uniform standard for the shape and size of the orifice box, but a square shape is often used, and the side length is generally 60 cm in the rat and the mouse is about 40 cm. (2) The number of holes is usually 16 holes at an early stage, and a 4-well plate is usually used now. (3) Animals often have more drill bits in the morning than in the afternoon. Therefore, try to experiment at a fixed time every day. (4) In this experiment, the specificity of the anesthetic drill caused by anxiety drugs (such as BDZ receptor inverse agonists) is more consistent, and some drugs lacking anti-anxiety effects (such as BDZ receptor antagonist flumaxi) Ni) can increase the exploratory drill bit. Therefore, when screening unknown drugs, it should be combined with other anxiety models for evaluation. Wood Pellet Machine,Wood Pellet Mill,Wood Pellet Press,Wood Pellet Line Shandong Longze Mechanical Equipment Co.,Ltd , https://www.pelletmachinefactory.com