Summary of laboratory glassware cleaning methods

Summary of cleaning methods
Ways to clean laboratory glassware
1. Manual cleaning: At present, the most important cleaning method in China relies on manual or hand-held tools for brushing or cleaning.
Advantages: universal cleaning method, high recognition, good adaptability, can adapt to various occasions with different pollution levels and different pollutants
Disadvantages: Subjective factors have great influence, quality is difficult to achieve stable; no recordable, traceable, and verifiable; in some cases, such as large size of utensils and large number of cleaning items, manual cleaning is labor intensive , bringing instability to quality control.
2, ultrasonic cleaning: based on the role of ultrasonic cavitation, when the ultrasonic wave suddenly breaks (closed stage) after the formation of bubbles can produce more than 1000 atmospheric pressure, this continuous generation of instantaneous high pressure strongly impacts the surface of the object, making the surface of the object And the dirt in the gap is quickly peeled off, so as to achieve the purpose of cleaning and purifying the surface of the object.

Advantages: Ultrasonic cleaning is good, especially for items with complex surfaces and small lumens. The aluminum foil test cavitation produces a powerful force that effectively removes dirt.

Disadvantages: Cavitation can damage the surface of the item


3. Washing machine cleaning: The cleaning solution enters the spray arm and the spray pipe under the pressure drive of the circulation pump, and the pressurized circulating water drives the spray arm to rotate to complete the cleaning of the cleaning articles. It is placed in the cleaning chamber and the cleaning process is carried out with an automated machine. In general, it is necessary to add an auxiliary cleaning agent for cleaning, and the steps of pre-washing-washing-rinsing-neutralizing-rinsing-drying can be completed. The cleaning process can be recorded, traceable, and the cleaning process can be verified.
Factors affecting the cleanliness of glassware
1, cleaning time:
Time and mechanical force: Generally, the longer the cleaning time, the better the cleaning effect, thus ensuring the cleaning effect.
Time and chemical reaction: Like mechanical force, the cleaning agent also needs a certain time to chemically react with the residue to dissolve, emulsify and decompose the residue.
2, cleaning temperature:
Temperature and surface tension of the washing water: Generally, the higher the temperature, the lower the surface tension and the enhanced wetting ability on the surface of the glassware
Temperature and cleaning materials and chemical reactions: Each cleaning program has the most suitable temperature, depending on the chemical reaction between the cleaning material and the cleaning agent. During this reaction, the proper temperature plays a good catalytic role.
Temperature and strong alkali cleaning agent: Containing KOH or NaOH, the higher the cleaning temperature, the better the cleaning effect, but the manual cleaning can not achieve the cleaning at a higher temperature.
Temperature and protein: The high temperature used at the beginning of the cleaning will solidify the protein, which is the main reason why the pre-cleaning process is carried out at room temperature.
3, cleaning mechanical force
The cleaning agent is driven by the circulating pump, and the cleaning liquid is sprayed to directly wipe the surface of the cleaning object by 360 degrees, thereby peeling off the pollutants on the cleaning object. For different cleaning articles, different flow rates and pressures are required to ensure vigorous and vigorous operation. At the same time of cleaning, please ensure that the cleaning is not destroyed due to excessive pressure.
4, cleaning agent
Alkaline detergents use metal saponification and emulsification, soaking and wetting mechanisms to remove metal surface oils such as saponifiable oils (animal and vegetable oils) and non-saponified oils (mineral oils), which are converted into fatty acids and ethanol. Alkaline detergents can also react with peptide bonds and carboxylates in proteins, converting them into amino acids and carboxylates, mainly for washing organic pollutants.
The acidic detergent mainly prevents and removes mineral deposits, and first converts the salts which are hardly soluble in water in the inorganic state into a soluble state, so that they are easily washed away. For example, lime precipitates produce carbon dioxide under the action of acid, and the dissolved state changes from insoluble to soluble, and the acid can also act as a neutralizing agent to effectively neutralize and remove the alkaline residue on the surface of the vessel to achieve the best washing effect.
5, cleaning water
Water is an important part of the washing process and is used as a dissolving detergent or rinsing liquid. It also provides mechanical and thermal energy during the washing process.

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