Culture strains prevent pollution
In the course of production, due to the infringement of the bacteria, it often leads to a reduction in production or even a loss. Therefore, the search for the cause of bacterial contamination is of great significance for improving the success rate of cultivation and improving economic efficiency. Under normal circumstances, the pollution rate of secondary and tertiary strains is below 5%. If this is beyond this range, we need to find out why. Cause bacterial contamination of the reasons are as follows: 1, sterilization is not complete culture material sterilization is not complete will cause bacterial contamination, generally 3-5 days can appear pollution. The sterilization is not complete due to the following several reasons: Improper operation of molds Mycelia and spores of the common bacteria can be killed by atmospheric pressure 100 °C, 6-8 hours or 121 °C, 0.5-2 hours. Anti-Allergic Agents,Chlorphenamine Tablets 4Mg NOUVASANT GROUP LTD. , https://www.nouvasant.com
2 Influence of the properties of the culture materials The types of materials needed for seed production, the bacterial content in the culture materials, the water content, and the pH can all affect the sterilization effect. When sterilizing, according to the different formulations of culture materials, select the appropriate sterilization time.
3 Bacteria Bottles (Bags) Implantation of Improper Bacteria Bottles (Bags) Stacking in sterilizers can affect the effectiveness of sterilization. There must be a certain gap in the discharge so that the steam can circulate and heat up.
The impact of 4 strains of bacteria or strains of bacteria in the selection of strains when the seed strainer or strainer bags. Plastic bags have a fast heat transfer rate, while glass bottles have a slow heat transfer rate. When using the same sterilization method for the same culture material, the bottle material is slightly longer than the bag material sterilization time. In addition, individual containers that are not tightly sealed or broken can also cause contamination with germs.
2. The inoculation operation is not standardized The inoculation process of edible fungi requires strict aseptic processing. If the operation is not standardized, contamination of culture materials with bacteria can be easily caused. The contaminated germs are generally the bacteria in the inoculation room, the bacteria on the surface of the bacteria bag, and the surface bacteria of the inoculation personnel.
3, bacterial species with bacteria for the expansion of the original species itself must be pure bacteria, so as to ensure the unity of the bacteria species. If the strain itself is not pure, it can cause batch pollution. In the batch of strains produced, the strains contaminated all appear to be contaminated, and the contaminated parts are located in the strain block where contamination occurs earlier and 3-5 times are inoculated. Days can appear. Therefore, in the production of seeds should be as far as possible the use of viable, suitable age of bacteria, strong resistance to the original species of fine strains.
4. Unclean culture room The culture material is not clean, the air does not circulate, the room is damp, and bacteria can also be infected. The pollution rate in this case is relatively low, but as the incubation time increases, the pollution rate will gradually increase. A lot of pollution appeared later, and it usually appeared after 10 days of inoculation.