Problems encountered in the preparation of monoclonal antibody ascites and countermeasures
1, less ascites or no ascites 2 The hybridoma cells or sensitizers were inoculated incorrectly, and they did not hit the abdominal cavity, but hit the skin. 3 The number of hybridoma cells inoculated is too small, too much or the cell state is not good. Generally, it is not suitable for about 500,000 cells. 4 It is recommended to use the mother or the mother who has given birth to prepare ascites. 1 The hybridoma for the preparation of ascites is extremely unstable. Before it hits the mouse, it loses its ability to secrete antibodies or loses its secretion capacity when it hits the abdominal cavity. 2 The sensitizer was used in sensitized mice. Frozen Fillet Tilapia,China Tilapia Fillet,Tilapia Fillet,Tilapia Fillet Frozen Fish Zhoushan City Shuangying Aquatic Products Co., Ltd.  , https://www.shuangying-aquatic.com
1 sensitization is incorrect, incorrect use of sensitizer, or sensitization time and time to inoculate hybridoma are too long, usually 7-14 days, depending on the abdominal condition of the mouse.
2, ascites has no potency
3 The number of hybridoma cells inoculated is too small, or the cell state is not good.
4 Solid tumors are formed due to incorrect injection methods and locations.