Experimental application of elevated cross maze and experimental video analysis system of ZS behavior

Elevated cross maze experiment application
Elevated cross maze experiment application
I. Experimental overview
The elevated cross maze examines the anxious state of animals by using animal exploration characteristics of new and different environments and fear of high open arms. The elevated cross maze has a pair of open arms and a pair of closed arms. Rodents tend to move in the closed arms due to their darkness, but they will move in the open arms out of curiosity and inquiry. Stimulating
Second, experimental instruments
The instrument model is ZS-3000, and the instrument consists of a cross labyrinth component, a camera device and a ZS animal behavior trajectory analysis system.
The cross labyrinth assembly has two opposite open arms, the material is made of advanced medical organic board, the contact surface is not reflective, and the alcohol can be used for cleaning and deodorizing;
ZS provides users with two tracking algorithms: grayscale and background subtraction, stable and reliable head, middle and tail tracking algorithms, and even microscopic organisms such as Drosophila larvae.
Third, experimental methods and applications
At the beginning of the experiment, the mice were placed into the maze from the central grid facing the closed arm, and the ZS behavioral software was recorded for data analysis.
The number of open arms and the duration of stay were negatively correlated with the anxiety of the rats. The fewer times the open arms were entered, the shorter the stay time, indicating that the anxiety of the rats was more serious.

ZS Behavioral Experimental Video Analysis System

  The ZS Behavioral Experimental Video Analysis System is a new generation of behavioral experimental analysis system that integrates video acquisition, behavior analysis, parameter recording and data statistics, which is newly launched by Beijing Zhongshi Di Chuang Technology Development Co., Ltd. The system captures analog or digital camera images through a flexible camera assembly, and analyzes and evaluates the effects with computer vision-related algorithms to obtain behavioral parameters of the animals. The system provides data interface to mainstream statistical software, and the experimental data can be exported and analyzed. At the same time, it has a statistical analysis report module for animal experiment analysis, which is convenient for users to quickly complete data collection and analysis and electronic archiving.
  The system adopts modular design, the user can define the experimental scene according to the needs, complete the diversified experimental steps through the regional setting and experimental condition setting, and automatically record the experimental data and experimental recording according to the scene definition and the area definition. It can capture video data through high-definition digital camera system, and analyze pre-recorded video files for demonstration and teaching.
  The unified design of the experimental platform, the installation is simple and convenient, easy to clean and also saves the user space occupied by the experimental equipment. The system can repeat measurements without affecting other behavioral analysis, and easily understand the adaptability of experimental animals to the new environment. It is often used to assess behavioral parameters such as exercise capacity, anxiety, and working memory. It can be widely used in experimental teaching and drug research work in medical colleges and research institutes of universities and colleges.
The system can complete the following behavioral experiments:
First, the opening event
Second, the elevated cross maze
Third, the Morris water maze
Fourth, the Zero Labyrinth
Five, T labyrinth
Six, Y maze
Seven and eight arm maze
Eight, shuttle experiment
Nine, avoid dark experiment
Ten, mouse jumping test
Eleven, mouse tail suspension experiment
Twelve, forced swimming experiment

Surfactants

We adopt a comprehensive classification method, which is divided by the ionicity of surfactants as follows which can all be provided by Insen Biotech: 1. Anionic surfactant: stearic acid, sodium dodecylbenzene sulfonate. 2. Cationic surfactant: quaternary ammonium compound. 3. Zwitterionic surfactants: lecithin, amino acid type, betaine type. 4. Non-ionic surfactants: fatty acid glycerides, fatty acid sorbitan (Span), polysorbate (Tween).

Surfactant Agent ,Carbomer 940 Powder,Surfactant Cosmetic ,Nonionic Surfactant

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