Chromatin immunoprecipitation study complete protocol

The genomic DNA of eukaryotes exists in the form of chromatin. Studying the interaction between protein and DNA in chromatin environment is the basic way to elucidate the mechanism of eukaryotic gene expression. Compared with traditional EMSA technology, chromatin immunoprecipitation (ChIP) can truly and completely reflect the regulatory proteins bound to DNA sequences. It is the best way to study the interaction between DNA and protein in vivo.

Chromatin immunoprecipitation assay (ChIP) is the best method to study the interaction between DNA and protein in vivo. Its basic principle is to immobilize protein-DNA complex in living cell state and randomly cut it into certain A small fragment of chromatin in the length range, and then immunologically precipitated the complex, specifically enriching the segment of the DNA fragment bound by the protein of interest, and obtaining information on the interaction between the protein and the DNA by purifying and detecting the target fragment. .

CHIP can not only detect the dynamic effects of trans-factors and DNA in vivo, but also study the relationship between various covalent modifications of histones and gene expression. Moreover, the combination of CHIP and other methods has expanded its range of applications: CHIP-on-chip method, which is based on the combination of CHIP and gene chip, has been widely used for high-throughput screening of specific trans-factor target genes; CHIP and in vivo footprint method Binding, used to find in vivo binding sites for trans-factors; RNA-CHIP is used to study the role of RNA in gene expression regulation. It can be seen that with the further improvement of CHIP, it will play an increasingly important role in the study of gene expression regulation.

As a professional solution provider for epigenetics, Aimeite recommends the most popular regular chromatin immunoassay kit from Epigentek and the most distinctive plant ChIP kit . In addition, in addition to the kit, Aimei can also provide ChIP-level antibodies related to the ChIP kit, which can be widely used in a variety of experimental applications including ChIP, IHC, IF, etc.

The most cost-effective tissue chromatin immunoprecipitation kit (ChIP) (p-2002) provides all the reagents needed for chromatin immunoprecipitation of cell samples. And the kit contains a positive control antibody (RNA polymerase II antibody), a negative control antibody (IgG of normal mice), GAPDH primer (can be used as a positive control to ensure that the reagents and procedures in the kit did not appear problem). In most mammalian cells in the growth phase, RNA polymerase II is enriched in the GAPDH gene promoter, ready to initiate transcription, so the promoter can immunoprecipitation with RNA polymerase II, but not normal Mouse IgG occurs. In this chromatin immunoprecipitation reaction, cells are coupled with formaldehyde to extract chromatin therein. The chromatin is appropriately interrupted and then added to the microwell to immunoreact with the antibody adsorbed on its surface. The DNA that specifically binds to the microwells is released from the antibody-capture protein-DNA complex, inverted, and purified by our company's specially designed high-speed spin column. The eluted DNA can be used directly for subsequent analysis. This kit is based on 96-well plate, the fastest kit in the market, the CHIP process is completely simplified, the operation is simple, easy to learn, the entire process is less than 5 hours, and the detachable 96-well The board mode allows researchers to choose manual or high-throughput analysis based on their needs.

(The picture on the left is a simple and convenient experimental procedure for the ChIP kit (p-2002)) (The right picture shows the excellent performance of the ChIP kit (p-2002))

As the most professional supplier of epigenetic products, Epigentek has uniquely launched a unique plant chromatin immunoprecipitation kit: EpiQuik Plant ChIP Kit (p-2014), which greatly expands the application of chromatin immunoprecipitation. The Plant Chromatin Immunoprecipitation (ChIP) kit provides all the reagents needed for chromatin immunoprecipitation of plant cell samples.

Amy Jet provides you with a one-stop ChIP-related experimental solution, in addition to the universal ChIP kit, as well as ChIP-level antibodies and recombinant proteins associated with the ChIP assay. There are more fields such as histone methylation ChIP kit (specific for monomethyl, bismethyl, trimethyl), histone acetylated ChIP kit, and ChIP kit for methylated DNA binding protein. The kit provides you with a one-stop service to take your research to the next level!

Epigentek Group is the world's leading developer and supplier of innovative technology and epigenetic research products. The company has developed a comprehensive product portfolio of more than 400 patented products to provide comprehensive system solutions for epigenetic research and new drug development. As the general agent of Epigentek Group's China region, Amy Jie introduced it to China as its regional agent in China and will provide customers with complete epigenetic solutions, including analytical kits for antibodies, antibodies and related Products such as inhibitors/antagonists. At the same time, as a Chinese agent or regional agent of many international famous brands, Aimei can provide you with a series of laboratory solutions. If you are interested in the above products, please call the free customer line 400-6800-868 to contact Aimei Technology Co., Ltd. for product information and complete experimental solutions.

Small 2D LiDAR Sensor

IT02S, is JRT new product in the early 2019, which is a single-point LiDAR sensor, also called tof distance sensor. With a micro size of 46x17x7mm, customers can widely use in many Laser Measurement Solutions. The lidar distance sensor can measure 12m short-range with high frequency up to 100hz. It's great for Unmanned Aerial Systems. If you need us send you data sheet and spec for this products, offering sample as well, pls tell us, thank you.

IT02S Mini Tof Sensor Module Diagram

Parameters of IT02S: