Effect of Chinese Cobra Cardiotoxin (CTX) on Pain and Memory
Effects of Chinese Cobra Cardiotoxin ( CTX ) on Pain and Memory ( This study is from Liu Huaqing, East China Normal University, etc. ) Abstract: The effects of Chinese cobra heart toxin (CTX) on the pain of adult Kunming mice were observed by hot plate method and acetic acid writhing method. The Y-type electric maze and shuttle box method were used to study the effect of CTX on the growth of Kunming. The effect of learning and memory impairment in rats was studied by using the jumping method to study the effect of CTX on learning and memory impairment induced by scutellarin in adult BABL/C mice. Morris water maze method was used to study the effect of CTX on adult C57BL/6J mice induced by scutellarin. The impact of memory impairment. The experiment found that CTX can significantly increase the pain closure caused by thermal stimulation, significantly reduce the number of writhing caused by acetic acid, and can significantly increase the number of correct responses in mice in the Y-maze experiment, reducing the retraining of mice in the shuttle box experiment. The training frequency of the standard and the reaction time of the mice can increase the latency of the mice in the platform jumping method, reduce the number of false reactions, and reduce the time of searching for the mice and the swimming distance in the Morris water maze experiment. Significant dose dependence. These results suggest that CTX has a good analgesic effect and an improvement in learning and memory impairment induced by emocorline in mice, which may be due to the similar biological effects of CTX and cobra neurotoxin (NT). . I. Experimental method 1.Y type electric maze method Kunming mice are male and female. The bottom of the Y-type electric maze is connected with 35v AC, and the mouse directly escapes to the left after being shocked by the electric shock. The side safety zone was the correct response, and the mice were trained 8 times in a row for 10 consecutive times to the standard of training to the society. After 24 hours, the mice that had been learned were randomly divided into groups. There was no significant difference in the number of trainings between the groups (P>0.05). 45 doses before the test, blank group and model group ip saline, positive group Ip 0.1mg/kg HupA, CTXl, CTX2, CTX3 group ip CTX 0.03, 0.06, 0.12mg/kg, except for the blank group 30min before the test, the other 5 groups were administered with 5mg/kg body weight of ip. Test memory scores, with the correct number of 10 fixed tests as the evaluation index. (Y-type maze video analysis system is provided by Shanghai Xinsoft Information Technology Co., Ltd., Y-maze is divided into food award type and electric stimulation type, and different experimental devices are required for different experiments). 2. Shuttle box method Kunming mice are male and female. The buzzer stimulation was used as a signal, and after 5 s delay, 35 v alternating current stimulation was applied to the ipsilateral side. The mouse responded correctly to the safe area within 5 s after the start of the buzzer. Training to the mouse is the correct standard for 4 times in 5 consecutive times. After 24 hours, the learned mice were randomly divided into 6 groups, and there was no significant difference in the number of trainings between the groups (P>0.05). The administration method is the same as the Y-type electro-maze method. The time required for each mouse to start from the buzzer to the safe area was recorded as the evaluation index by the number of trainings required for the reaction and the re-training to the next four times. (The shuttle box video analysis system is provided by Shanghai Xinsoft Information Technology Co., Ltd., and the SuperAPAS shuttle box video analysis system developed by Shanghai Xinsoft adopts the most advanced recognition algorithm. Different recognition algorithms are used according to different experimental environments, and other shuttles are used. Compared with the video analysis software, the SuperAPAS shuttle box video analysis system has high accuracy, can adapt to different experimental environments, and has many test indicators. 3. Jumping method BABL/C mice were male and female, and the mice were trained to stand on the platform for 3 min without contacting the bottom copper grid as the standard of learning. After 24 hours, the learned mice were randomly divided into groups, and there was no significant difference in the number of trainings between the groups (P>0.05). The administration method is the same as the Y-type electro-maze method. The time when the mouse first contacted the bottom copper grid in the test was used as the incubation period. The number of times the mouse was exposed to the copper grid within 5 minutes was the number of errors, and the latency and the number of errors were used as evaluation indexes. (The platform is provided by Shanghai Xinsoft Information Technology Co., Ltd., and the instrument has been adopted by universities and medical institutions, and the effect is good) 4.Morris water maze method The Morris water maze has a diameter of 74 cm and a depth of 48 cm. The diameter of the standing platform of the mouse is 7.3 cm, the height of the platform is 24 cm, and the water depth during the experiment is 25 cm. The water was adjusted to opaque with fresh milk powder [ys). The experiment used C57BL/6J mice, half male and half, and the mice were placed on the platform for 30 s. The mice were placed face to face in the water, until the platform was found and stayed on the platform for 30 s. Each mouse allowed a maximum swimming time of 120 s, and over 120 s was screened to the platform with a sieve for 30 s. Each mouse was trained 4 times a day (with 4 different quadrants in the labyrinth as the starting point), each interval of 30 s. The time of the mouse search was recorded as the academic achievement. After 5 days of continuous training, the mice were divided into 6 groups according to their academic scores, 10 in each group. There was no statistical difference in the time between the groups (P>0.05). The administration method is the same as the Y-type electro-maze method. Start all the mice from the same starting point (as opposed to the location of the platform), record the time of the search, and swim. All data were expressed as x S, and the difference between groups was statistically analyzed by t test. (Morris water maze video analysis system is provided by Shanghai Xinsoft Information Technology Co., Ltd., Shanghai Xinsoft's water maze occupies the market's big wall. This water maze uses the most advanced ABS engineering plastics, and materials such as stainless steel and iron sheet. Compared with the characteristics of portability, toughness, high thermal insulation coefficient, no rust, easy to clean, etc., it has been favored by researchers. Its unique Supermaze video analysis system adopts the domestic top identification algorithm and improves. Accuracy of the experiment) III. Experimental results The correct number of mice in the Y-type electromass experiment of CTX 0.06mg/kg and 0. 12mg/kg group was significantly higher than that of the model group (P<0.O1), and the correct number of mice in the CTX 0, 12mg/kg group. Similar to the positive control group (P>0.05) o The response time of the CTX 0.06mg/kg and 0.12mg/kg mice in the shuttle box experiment and the number of training required for memory reproduction were also significantly lower than the model group (P<0.O1), of which CTX 0.12mg/ The memory scores of the mice in the kg group were comparable to those in the positive control group (P>0.05). In the platform test, the mice in the CTX 0.06mg/kg and 0.12mg/kg groups were significantly higher in latency than the model group (P<0.O1), and the number of errors was significantly lower than the model group (P<0.O1). The memory scores of mice in the CTX 0.12mg/kg group were comparable to those in the positive control group (P>0.05). The time between the CTX 0.06mg/kg and 0.12mg/kg mice in the water maze experiment and the swimming distance were both The memory performance of the mice in the CTX 0.12 mg/kg group was significantly lower than that in the positive control group (P>0.05). The above results all showed dose dependence. Stomach Tube,stomach tube sizes,Feeding Tube,tube feeding Anesthesia Medical Co., Ltd. , https://www.jssinoanesthesia.com