Molecular Devices uses MiniMax imaging system to evaluate the effects of compounds on Hela-GFP cell proliferation and morphology

Since the 20th century, environmental issues have received increasing attention from society. Thousands of chemical substances have been studied and reported. The release of these compounds into the environment will likely result in long-term, adverse effects on wildlife and humans.

Recently, researchers at Huazhong Agricultural University published an article entitled "Evaluation of the effects of chemicals on the proliferation and morphology of Hela-GFP cells by label-free screening experiments" in "Environmental Science & Technology Letters" (formerly English title: A Reagent-Free Screening) Assay for Evaluation of the Effects of Chemicals on the Proliferation and Morphology of HeLa-GFP Cells). The study utilized Molecular Devices' MiniMax imaging system for label-free screening experiments in which inoculated HeLa cells were treated with a variety of compounds to assess the effects of compounds on cell proliferation and morphology based on dose- and time-dependent changes.

The experimental results demonstrate that label-free screening experiments can support long-term or real-time compound effect monitoring without any labeling of cells, and can be used as an effective new method for chemical screening without additional manipulation and damage to cells.

The graph shows total cell fluorescence, cell number and MTT activity after exposure to various concentrations of various compounds: minimum observed effect concentration (LOECs) and half effect concentration (EC50)

Click to download the original English text: A Reagent-Free Screening Assay for Evaluation of the Effects of Chemicals on the Proliferation and Morphology of HeLa-GFP Cells.pdf

Click to learn about the MiniMax imaging system