Human thioredoxin domain containing protein 6 (TXNDC6) ELISA kit procedure
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Brand: Keshun Biological Use: Scientific research and other fields of scientific research, can not be used for clinical diagnostic product advantages:
1, the original imported antibodies ---- efficient, sensitive, specific
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3, the specification of the package operation ---- uniform adsorption, good adsorption, low blank value, high transparency at the bottom of the hole
4, advanced optimization program ---- high repeatability, reliability
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6, high standard technical service requirements: efficient, professional, normative,
7, a wide range of applications: plasma, serum, tissue homogenate, cell culture supernatant, urine and other types of samples
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Human thioredoxin domain containing protein 6 (TXNDC6) ELISA kit procedure
1. Remove the required slats from the foil pouch after equilibrating for 20 min at room temperature. The remaining slats are sealed back to 4 °C with a ziplock bag.
2. Set standard and sample wells, and add standard concentration of 50μL to each standard.
3. Add 50 μL of the sample to be tested to the sample well; blank holes are not added.
4. In addition to the blank wells, add 100 μL of horseradish peroxidase (HRP)-labeled detection antibody to each well of the standard wells and sample wells, seal the wells with a sealing membrane, and incubate in a 37 ° C water bath or incubator. 60min.
5. Discard the liquid, pat dry on the absorbent paper, fill each well with the washing solution (350 μL), let stand for 1 min, remove the washing solution, pat dry on the absorbent paper, and repeat the washing 5 times (can also be washed with a washing machine) board).
6. Add 50 μL of substrate A and B to each well and incubate at 37 ° C for 15 min in the dark.
7. Add 50 μL of stop solution to each well, and measure the OD value of each well at a wavelength of 450 nm within 15 min.
(1) Reagents should be stored according to the label instructions and returned to room temperature before use. Standards after dilution should be discarded and cannot be stored.
(2) The slats not used in the experiment should be immediately put back into the packaging bag and sealed to prevent deterioration.
(3) Other reagents that are not used should be packaged or covered. Do not mix reagents of different batches. Use before warranty.
(4) Use a disposable tip to avoid cross-contamination. When aspirating the stop solution and the substrate A and B, avoid using a metallized sample applicator.
(5) Dispose of the washing liquid in a clean plastic container. Mix all the ingredients and samples in the kit thoroughly before use.
(6) Wash the enzyme plate should be fully patted dry, do not put the absorbent paper directly into the enzyme standard reaction well to absorb water.
Human thioredoxin domain containing protein 6 (TXNDC6) ELISA kit free proxy
The test materials and reagents of the entrusted unit shall be mailed by express mail, and if required for cryogenic requirements and fixed requirements, transported by cryopreservation and immobilization of human thioredoxin domain containing protein 6 (TXNDC6) ELISA kit. To ensure the safety and reliability of the experimental items.