Fully automated solution for compound storage and analytical plate preparation
Zhejiang Haisheng Medical Device Co., Ltd , https://www.hisernmedical.com
Fully automated solution for compound storage and analytical plate preparation
Alberto Bresciani 1 , Stefan Mauch 2
1. IRBM Science ParkSpA, ViaPontina km 30600,0040 Pomezis(RM), Italy
2. Hamilton Bonaduz AG, Via Crusch 8, Bonaduz, Switzerland
Introduction
Founded in 2010, the National Compounds Collection and Chemical Screening Center (CNCCS) is a public-private partnership jointly managed by the Italian National Research Council (CNR), the Royal Dionysita Institute of Advanced Studies (ISS) and the IRBM Science Park. Medical research. With more than 50,000 compounds, the center provides internal drug discovery screening and external customers with the preparation of compound sample plates for a variety of screening assays. The entire process of compound storage and analytical plate preparation is automated by an integrated Hamilton system.
The system is designed to store cryotubes and microplates and handle all compound management activities including recombination, analytical plate preparation, Hits picking and QC, etc. The centralized processing of these functions helps to optimize resources and reduce costs. Continuous and effective control of the quality of the compound ensures the reliability of the screening results.
 Figure 1: System overview
system introduction
The system consists of an ASM storage system (A) manufactured by Hamilton Storage Technologies and a server (B) connected to Rack Runner (C). Rack Runner transfers compounds, Source boards, and analysis boards between storage devices and surrounding instruments. These peripherals include a Hamilton STARplus liquid handling station (D), an ETS biological system ATS-100 acoustic dispenser (E), a ThermoFisher Scientific ALPS 3000 automatic sealing machine (F), and a Fluidix XDC -96 PRO Automatic capping/uncovering device (G) and a Brooks XPeel automatic film removing machine (H).
Figure 2: System schematic
work process
The compound stock was stored in a 0.75 ml Thermo Source tube in a -20 ° CASM system. Once a year, the compound is dispensed into the Source plate (see Figure 3). The analytical plates were then prepared from the Source plate using acoustic transfer methods as required by analytical experiments. Compound dispensing once a year is an ongoing process of the year. The corresponding stock cryotube rack was selected from the ASM storage system and then melted in the ASM server and transferred to the Hamilton STARplus liquid handling system via the Rack Runner. There, the stock solution was centrifuged by a centrifuge and the cap was removed by a Hamilton cap/decap. The stock solution was then dispensed into a 96-well Source plate. Four 96-well Sources can be combined into a single 384-well Source plate. The Hamilton capping/uncovering device then re-caps the storage tube and transfers it back to the ASM storage system. The Source board will be sealed and stored in the ASM system for later use.
When the analysis board needs to be dispensed, the corresponding Source board will be picked from the ASM system, melted in the server, and then transferred to the acoustic pipette where it is dispensed to the analysis board.
The core strategy of the process is to maximize the time that compounds are stored in the same location. The goal is to maintain a backup of the specific microplate, whether it is a Source plate or a newly prepared plate that is left in a year to verify the experimental results. Moreover, this process maintains consistency in the consumption of compounds in the library, thus ensuring the longest use time without the need to re-synthesize individual compounds.
Figure 3: System overview
Technical core
In order to meet all of the customer's requirements, Hamilton has customized the integrated system with different supplier products (see Figure 2 to understand the specific structure and layout details of the system), to achieve the best match of the experimental process, and through the connection The computer in the local LIMS system controls the entire system.
The core of the system is the ASM storage system and server, and through the Rack Runner connection system core and STARplus and other equipment, the cryotube and microplate are stripped, sealed and acoustically separated.
Microlab ® STARplus uses Hamilton's CO-RE technology to operate all pipetting steps to ensure precise liquid transfer.
Figure 4: Hamilton Microlab STARplus automated liquid handling workstation