Introduction of the method for determining the freezing strength of gelatin by texture analyzer

Gelatin, which has no fixed structure and relative molecular weight, is partially degraded by collagen in the connective tissue of animal skin, bone, sarcolemma, muscle, etc. to become white or pale yellow, translucent, micro-glossy flakes or powders. It is a colorless, odorless, non-volatile, transparent and hard amorphous material. It is soluble in hot water and insoluble in cold water, but it can be slowly swelled and softened. Gelatin can absorb water equivalent to 5-10 times by weight.

Gelatin is one of the most important natural biopolymer materials and has been widely used in the food, pharmaceutical and chemical industries.

1. Equipment and accessories

Equipment : UTA multi-function texture analyzer or RTA-gel gel strength tester

P/0.5inch probe : stainless steel piston cylinder with diameter of 12.700 mm ± 0.013 mm

Frozen bottle : capacity 150 mL, inner diameter 59 mm, height 85 mm

2 principle

Under the specified conditions, when pressed into a cylinder with a diameter of 12.7 mm by 4 mm below the jelly surface containing 6.67 % gelatin solution, the applied force represents the freezing strength in Bloom g.

3 analysis steps (reference standard download: gelatin freezing strength GB6783 - 2013 national standard)

Prepare 120 mL of 6.67% sample solution in a frozen bottle, cover it, and cool it in a low temperature tank at 10 °C ± 0.1 °C for 16 h to 18 h. Remove the freezing bottle from the constant temperature water tank and place it on the round table of the texture analyzer or the freezing strength meter. The target mode selection distance is 4 mm, the test speed is selected to be 0.5 mm/s or 1 mm/s, and the freezing strength is measured. The sample test needs to be completed within 2 minutes.

4 result calculation

The measured freeze strength values ​​are read directly from the texture analyzer or the freeze strength tester in units of Bloom g. The result is taken as an integer, and the arithmetic mean of the parallel measurement results is taken as the measurement result. The absolute difference between two independent results obtained under repeatability conditions must not exceed 10 Bloom g

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