Introduction to the overall solution and method package for pesticide residue detection in tobacco

Introduction to the overall solution and method package for pesticide residue detection in tobacco
Thermo Fisher Scientific Triple Quadrupole Qualitative
1. Introduction to the method package
The method package is a simple instrumental application process for the customer's needs by the application department of Thermo Fisher Scientific and Mass Spectrometry. The compounds involved in the method package are common compounds that can be detected on GC/MS, such as pesticide residues. Cycloaliphatic hydrocarbons, polychlorinated biphenyls, polybrominated biphenyls, polybrominated diphenyl ethers, phthalates, and the like. The role of the method package is to enable customers to use the instrument faster and easier, and get started as soon as possible.
The method package includes injection method, data processing method (TraceFinder method folder), related application articles, related standards, column information, pre-processing methods, data files, etc. The customer can directly call the injection method and data processing method to complete the compound. Qualitative and quantitative analysis.
Since the TSQ 8000 Evo uses the T-SRM injection method instead of the time-sharing method, the original injection method can be called directly, even if there is a slight deviation in retention time, it will not affect the final result. In addition, the database that comes with TraceFinder software can directly edit the data processing method. The database includes the name of the compound, ion pair, collision energy, quantitative ion, qualitative ion, CAS number and other information. At the same time, TraceFinder software can automatically correlate the method files that generate TSQ 8000 Evo according to the data processing method. This way, TraceFinder can be used to directly generate data processing methods and partial injection methods. The entire process is automated, requiring little operator input of any operational information.
Method package (Method Kit)

2. Introduction to the instrument
TSQ 8000 Evo triple quadrupole series gas mass spectrometer

With a consistent lead in gas chromatography triple quadrupole mass spectrometry, Thermo Fisher Scientific has reinvented the TSQ 8000 after the introduction of the TSQ 8000 Evo, a highly efficient GC/MS. /MS delivers never-ending productivity with exceptional sensitivity, ultra-fast scanning speeds, and simple MSMS capabilities to meet the most demanding quantitative analysis requirements, providing the foundation for food safety, environmental analysis, forensic and pharmaceutical applications.
Main features of TSQ 8000 Evo :
• AutoSRM function automatically optimizes secondary ion pair information;
• Timed Retention Time SRM function (T-SRM) and Timed Retention Time SIM function (T-SIM) make high-throughput detection possible, and the optimization method sets parameters without assigning time periods;
• The latest TraceFinder software, which provides the latest technology platform and user-friendly chromatographic analysis software, can directly call the database information to automatically establish data processing methods and injection methods;
• Up to 800 MRM/s scanning speed, even without extreme loss of sensitivity under extreme high-speed scanning conditions;
• Method bridges can quickly convert mass spectrometry methods from other instruments or other companies;
• Replacing the entire ion source while the mass spectrum is not venting, saving on routine system maintenance and simplifying operation;
• Double filament design with filaments on the same side;
• Double heating zone on the ion source to effectively remove the contamination of the ion source by the substrate and save maintenance time;
• Curved pre-quadpoles effectively remove neutral noise, reduce background and increase sensitivity.
III. Introduction to compounds
The tobacco standard was officially implemented in February 2014.
Table A.1 Chinese and English names and CAS numbers of 168 pesticides and 2 internal standard substances

Thermo Fisher Scientific can supply all pesticide residues in the tobacco standard at a concentration of 50 ppm and the solvent is acetone or methanol for customer convenience.
4. Relevant regulations and policies
On January 15, 2014, China National Tobacco Corporation issued the industry standard YQ/T 47.1-2014 for the determination of various pesticide residues in tobacco and tobacco products. The first part is the method of serially detecting phenolic pesticide residues. The standard was officially implemented on February 10, 2014.
V. Sample preparation
Pretreatment according to YQ/T 47.1-2014 Determination of multiple pesticide residues in tobacco and tobacco products
Extraction: Weigh 2 g of sample into a 50 mL capped centrifuge tube to the nearest 0.01 g, add 10 mL of water (4.1), shake with a hand-held centrifuge tube until the sample is thoroughly mixed with water, and let stand for 10 min. Pipette 10 mL of acetonitrile (4.2) and 0.1 mL of mixed internal standard working solution (4.11.4) into the centrifuge tube and place on a vortex mixing shaker (5.2) for 2 min at 2000 r/min. The tube was frozen and stored at (-18 ° C ~ -20 ° C) for 10 min and then taken out. Add 5 mL of toluene (4.3), 4 g of anhydrous magnesium sulfate (4.4), 1 g of sodium chloride (4.5), 1 g of sodium citrate (4.6), and 0.5 g of disodium hydrogen citrate (4.7). Immediately, the centrifuge tube was shaken vigorously to prevent agglomeration, and then placed on a vortex mixing shaker (5.2) for 2 min at 2000 r/min, and then centrifuged at 4000 r/min for 5 min. Collect the supernatant.
6. Instruments and equipment
Instrument: Thermo ScientificTM Gas Chromatography Triple Quadrupole Tandem Mass Spectrometer TSQ 8000 Evo.
Equipment: vortex mixing shaker, centrifuge, nitrogen blow concentrator.
Column: TR-Pesticide II (30 m*0.25 mm*0.25 μm with 5 m precolumn) capillary column (Thermo ScientificTM). The C10 retention time is 18.90 min.
Seven. Use of the method package
1, injection method use: directly use the file ending in the method package meth, for injection analysis.
Different instrument configurations may vary slightly. You can refer to the screenshot below. The first time you use this method, you can use the Time in the MS method.
Window is set to 4–5 min. After determining the retention time of each compound, change the Time Window to 1–2 min.

2. Copy this method file to C:\TraceFinderData\32\InstrumentMethods.
3, the data processing method is established: copy the folder in the method package to C:\TraceFinderData\32\Methods, the method can be opened directly in the TraceFinder software, this method includes the data processing method and the injection method.

Select the Tobacco pesticide from the drop-down menu in the Instrument method to save the Master Method.

4. Associate the data file completed in step 1 with the TraceFinder method to get the complete data processing method. The whole process does not need to input compound information, ion pair information, select ion pair information to find compounds, all steps are automatically completed, one point can be, greatly improving the analysis efficiency and simplifying the method flow.

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