How to characterize unknown samples using standard materials in liquid chromatograph analysis
When using the liquid chromatograph to analyze the sample, there are many factors affecting the solute migration in the liquid chromatograph, and the retention values ​​of the same component under different chromatographic conditions vary greatly, even under the same operating conditions. The retention values ​​of the components on different columns can also vary greatly, so liquid chromatographs are more difficult to characterize than gas chromatographs, which is also possible with liquid chromatography-mass spectrometry (LC-MS) systems. The reason for the rapid development. Characterization of unknown compounds using standard material sample samples is the most commonly used qualitative method for liquid chromatography analysis. The principle of this method is basically the same as that of gas chromatography. Since each compound has the same chromatographic conditions, such as mobile phase composition, column and column temperature, and its retention value has certain characteristics, it can be characterized by its retention value. If the test compound is consistent with the retention value of the standard sample under the same chromatographic conditions, it can be preliminarily considered that the test compound is the same as the standard sample. If the retention of the test compound after multiple changes in the composition of the mobile phase is still consistent with the retention of the sample of the standard sample, it can be further confirmed that the test compound is indeed the same compound as the standard sample. Hydrophilic Introducer Sheath Kit Hydrophilic Introducer Sheath,introducer sheath,Hydrophilic,arterial sheath introducer Anesthesia Medical Co., Ltd. , https://www.jssinoanesthesia.com