High-performance liquid chromatography column common troubleshooting methods
After the chromatographic column of the liquid chromatograph is used for a period of time, certain common faults will occur. If it is not judged and solved well, it will affect the use of the column and affect the analysis result of the sample. The chromatographer of Shandong Luchuang Analytical Instrument Co., Ltd. summarizes how to correctly judge and eliminate the common faults of HPLC columns, and hopes to help you use the liquid chromatograph correctly. Several common fault judgments and troubleshooting methods for high performance liquid chromatography columns: 1. When the column pressure is higher than the normal value, there are three cases: (1) blockage of the column end filter (2) long-term use of the column end stationary phase plate (3) analysis of samples of biochemical, dye and other easily contaminated stationary phases. Corresponding exclusion methods: (1) Remove the filter with ultrasonic cleaning with nitric acid (2) Excavate the plated portion and repair the column end (3) with a guard column. 2. When the column pressure is lower than the normal value, it may be leaking at a joint. Use high pressure to find the leak, remove the column and tighten it properly or lining the PTFE film. 3. When the number of trays drops sharply, there are two cases: (1) overage service (2) column is contaminated. Corresponding exclusion methods: (1) column regeneration or column replacement (2) cleaning the column. Through the above description of several fault analysis and elimination methods of liquid chromatography column, whether there is a new understanding and understanding of the use of liquid chromatography column, if you have any questions, please call Shandong Luchuang Analytical Instrument Co., Ltd., we will For detailed answers to your questions, you can also contact the staff online at http:// Shandong Luchuang Instrument is committed to promoting the quality of domestic analytical instruments. The use of quality and service for the majority of users is the "duty" and honor of Luchuang Instruments! With the improvement of people's living standards, people pay more and more attention to the importance of physical health. More and more people will use their spare time to exercise, run, ride, climb mountains and so on.
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